Figure 4. Effector cytokine potential is acquired alongside CD200R expression in vivo.

Dot plots show cytokine⁺ gated CD4 cells ex vivo (-P/I) and after recall with pdbU + Ionomycin (+P/I, 5h); gates are based on unstimulated controls (-P/I). A. Infection with S. mansoni (8wk) increased CD200R⁺ IL-4⁺ CD4 cells in MesLN from 0.017±0.019% to 3.39±1.36% and 83±4.14% of IL-4⁺ cells were CD200R⁺, while CD200R^– TNFα⁺ CD4 cells decreased from 47.5±4.66% to 30.0±0.99%. (n = 3, p = 0.01). B. Infection with S. enterica increased IFNγ⁺ CD4 cells in spleen from 1.9±0.65% to 31.1±4.4% and 49.5±9.86% of IFNγ⁺ cells were CD200R⁺, while CD200R^– TNFα⁺ CD4 cells decreased from 19.6±2.31% to 15.1±1.56%. (n = 5, p = 0.01). Results are representative of at least 3 (A, n = 3 mice/group) and 2 (B, n = 5 mice/group) independent experiments.