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. 1990 Sep 25;18(18):5419–5423. doi: 10.1093/nar/18.18.5419

Site specific functionalization of oligonucleotides for attaching two different reporter groups.

S Agrawal 1, P C Zamecnik 1
PMCID: PMC332219  PMID: 2216715

Abstract

The synthesis of an oligonucleotide functionalized to attach two different reporter groups at specific internucleotide linkages is described. To incorporate the amine specific reporter group the internucleotide linkage is modified to phosphoramidate (N-1-aminoalkyl) and for a thiol specific reporter group the internucleotide linkage is modified to a phosphorothioate diester. The synthetic cycle for introducing the modified internucleotide linkages at specific sites can be carried out using an automated DNA synthesizer. Combination of reporter groups have been attached successfully.

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Selected References

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