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. 2012 Apr 6;90(4):708–714. doi: 10.1016/j.ajhg.2012.03.005

Figure 2.

Figure 2

Subcellular Localization of ROGDI in Transfected HEK 293 Cells and Blood Mononuclear Cells

(A–F) The immunostaining of ROGDI-transfected HEK 293 cells was performed with a ROGDI polyclonal antibody incubated in the presence of a permeabilizing reagent (0.2% saponin). ROGDI labeling (A) was revealed with an Alexa Fluor-488 goat anti-rabbit secondary antibody (green). Nuclei (B) were stained with DAPI (blue). The merged picture (C) shows the ROGDI-antibody staining (green) together with nuclei staining (blue). As a control, the same experiment was performed with the secondary antibody in the absence of ROGDI antibody (D, E, and F).

(G–R) The same ROGDI antibody was used for immunolocalization of native ROGDI in blood mononuclear cells (green signal in G, K, and O). Colabeling was performed with a LAMIN A monoclonal antibody (red signal in H, L, and P). Nuclei (I, M, and Q) were stained with DAPI (blue). The partial colocalization of ROGDI with LAMIN A is shown in (J), (N), and (R). Cells were observed by confocal microscopy. Three cell sections from the middle to the top of cells are shown (G–J, K–N, and O–R, respectively). White scale bars represent 10μm.