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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Dev Biol. 2012 Feb 25;365(1):219–228. doi: 10.1016/j.ydbio.2012.02.027

Figure 4. Phosphorylation of Numb at serines 48 and 52 regulates its localization, but not function.

Figure 4

(A–B) NumbS2A localized uniformly throughout the entire cortex of the telophase neuroblast, while NumbS2D was enriched in the basal cortex. (n = 17 and 19, respectively) (C–D) Expression of NumbS2A or NumbS2D driven by Ase-GAL4 suppressed the supernumerary neuroblasts in lgl334/3644 mutant brains. (n = 8 and 9, respectively) (E) Quantification of the number of type I neuroblasts per brain lobe is shown for expression of the transgenic Numb proteins by Ase-GAL4 in lgl334/3644 mutant brains. (F–I) Similar to full-length Numb, expression of NumbS2A or NumbS2D suppressed the supernumerary neuroblasts induced by expression of aPKCcaax driven by Wor-GAL4, while expression of NumbΔAB did not have an effect on the supernumerary neuroblast phenotype. (n = 8 per genotype) Brains were stained with the indicated markers. White arrows indicate the type I neuroblasts. Scale bars are 10 μm (A–B) and 25 μm (C–I).