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. Author manuscript; available in PMC: 2013 Apr 6.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Mar 7;420(2):325–330. doi: 10.1016/j.bbrc.2012.02.158

Fig. 1. SAHA and TSA restore DLC1 expression in prostate cancer cells.

Fig. 1

(A): Methylation of DLC1 promoter in 22Rv1, LNCaP and C4-2-B2 cells. P control, universal methylated human DNA; M, methylated; U, unmethylated. (B): Chromatin immunoprecipitation analysis of Histone H3 acetylation on the DLC1 promoter. 22Rv1, LNCaP, and C4-2-B2 were treated with TSA or SAHA. Two sets of primers spanning different DLC1 promoter regions were used, which yield products of 124bp and 215 bp in length, respectively. M, DNA marker. (C): Restoration of DLC1 expression lines 22Rv1, C4-2-B2 and LNCaP cells by SAHA and TSA. DLC1 expression level was measured by real-time PCR.