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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2012 Mar 1;83(11):1552–1561. doi: 10.1016/j.bcp.2012.02.019

Figure 2.

Figure 2

Figure 2

Figure 2

A) Displacement by MRS5449 of radioligand binding to membrane preparations from CHO and HEK293 cells stably expressing hARs. Results are from one experiment representative of three independent experiments performed in duplicate. The Ki values from three experiments are listed in Table 1. B) Rightward-shift by MRS5449 of the concentration-response curve of the A3AR agonist Cl-IB-MECA in inhibition of forkolin-stimulated cyclic AMP production in CHO cells expressing the A3AR. C) Schild analysis of the effect of the fluorescent antagonist MRS5449 on inhibition of cyclic AMP accumulation induced by the A3AR agonist Cl-IB-MECA in CHO cells expressing the A3AR. The KB value was calculated to be 4.8 nM.