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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochem Pharmacol. 2012 Mar 1;83(11):1552–1561. doi: 10.1016/j.bcp.2012.02.019

Figure 3.

Figure 3

Fluorescence ligand binding experiments with compound 1 – 6 and MRS5449 with FCM. hA3AR-expressing CHO cells were incubated with fluorescent small molecule conjugates were used in a concentration approximately two times higher than its Ki values, and mean fluorescence intensities (MFI) of the cell-small molecule conjugates were measured with FACSCalibur flow cytometer. MFI values were converted into MESF values using QuickCal program v. 2.3 after correcting for autofluorescence. Percentages above each column show the brightness of each compound using MRS5449 set at 100%. Results are expressed as mean ± S.E. (n=3).