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. Author manuscript; available in PMC: 2013 Apr 9.
Published in final edited form as: Biomacromolecules. 2012 Mar 22;13(4):963–973. doi: 10.1021/bm300332w

Figure 2. Serum proteins are required for initial anchorage of human mesenchymal stem cells to PEGDA superporous hydrogels.

Figure 2

Representative live (green)-dead (red) pseudocolor stained images for hMSCs seeded within PEGDA SPHs and cultured in complete DMEM for 24 hours. (a) Cells with extended morphology toward nearby cells as marked by arrows could be observed. (b) Magnified region from (a) to show the cell-cell projections where the arrows are in approximately the same location. (c) When the culture was deprived of serum for the following 24 hours (total 48 hour culture), the cells remained viable. (d) However, when the hMSCs were seeded in PEGDA SPHs and cultured directly in serum-free medium for 24 hours, the cells did not survive (red staining), as confirmed by the absence of live cells (no green) within the live-dead staining image. The scale bar is 100 μm in each image.