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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Dev Biol. 2012 Feb 18;365(1):110–117. doi: 10.1016/j.ydbio.2012.02.013

Figure 2. TGFβ regulates migration in sclerotome associated mesenchyme in culture.

Figure 2

(A) Sclerotome and associated mesenchyme was isolated from E11.5 Tgfbr2fl/fl embryos and infected with adenovirus Cre (Ad-Cre) to delete Tgfbr2 or with adenovirus GFP (Ad-GFP) as a control. Loss of Tgfbr2 resulted in reduced basal migration. ***, P<0.001. RT-PCR in the upper panel shows the deletion of the Tgfbr2 allele using Ad-Cre, 100 MOI. (B) The migration assay was performed on sclerotome and associated mesenchymal cells isolated from E11.5 wild type embryos and treated with or without TGFβ in the bottom well. TGFβ stimulated migration. ***, P<0.001. (C) Cells were isolated from Col2aCre+;Rosa26/+ mice and used in the migration assay with TGFβ in the bottom well. Sclerotome derived cells were stained blue with X-gal, unstained cells represent sclerotome associated cells. The total nonmigrated cell population demonstrated both stained and unstained cells. Migrated cells at the bottom of the chamber were not stained. Images were selected from several wells for both total and migrated cells. Magnification: 40×.