Figure 1.
In vivo local perfusion of the L5 DRG with S1P increases mechanical sensitivity of the ipsilateral hindpaw. Perfusion with 100 μM S1P or vehicle (ACSF) at 1μL/hour occurred during the first 7 days (shaded area). The baseline plotted on day 0 is the average of 3 measurements made on preceding days. Data were normalized to the average baseline value for all animals, which did not differ between the two groups. *, significant difference between S1P and ACSF groups (2 way ANOVA with Bonferroni posttest). In all figures the significance level is indicated by the number of symbols (see Methods). N = 4 animals per group.