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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: J Control Release. 2012 Jan 15;159(1):104–110. doi: 10.1016/j.jconrel.2012.01.007

Figure 6. The nanocomplex-mediated gene delivery in different lymphoma/leukemia cells.

Figure 6

Cultured Jurkat leukemia/lymphoma cells, K562 myeloid leukemia cells, and Karpas 299 anaplastic large T-cell lymphoma cells were pre-treated with polybrene (6μg/mL) and then transfected with Lipofectamine containing the pMax-GFP reporter or the PBAE polymer (> 7 kDa)-plasmid nanocomplexes as described above. Plasmid pMax-GFP alone was used in control experiments (−). The total GFP-expressing cells were quantified by flow cytometry 48 hr post-transfection (%). The percentages of cells with different levels of GFP expression were also calculated (light green zone: <101; intermediate green: 101 to 102, and darker green: >102). Cellular GFP expression was also confirmed by fluorescent microscopy.