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. Author manuscript; available in PMC: 2013 Apr 25.
Published in final edited form as: Neurosci Lett. 2012 Mar 7;515(1):12–17. doi: 10.1016/j.neulet.2012.02.092

Fig. 1. Isolation of cultured neurons for detection of TxA2R and TRPV1 gene expression.

Fig. 1

Single cells or media-only controls from NG and DRG cultures were isolated and placed directly in RT-PCR buffer which contained primer sets for NFM, TxA2R (TP), and TRPV1. After RT-PCR, 1 μl of sample was taken for a second round of PCR and placed in tubes which separately contained nested primers for each specific gene of interest. The PCR products were visualized by gel electrophoresis.