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. Author manuscript; available in PMC: 2013 Apr 10.
Published in final edited form as: J Control Release. 2012 Jan 20;159(1):111–119. doi: 10.1016/j.jconrel.2012.01.010

Fig. 1.

Fig. 1

Polymer/pDNA complexation was assessed using a Gel Retardation assay. An equal volume solution of a) p(TETA/CBA) or b) mixture of p(TETA/CBA)/TCP (75% TCP) was added to 500ng pDNA in HEPES buffered saline and allowed to incubate for 30 min before being loaded on a 1% agarose gel, stained with EtBr, and then run for 30 min in TAE buffer. Total polymer amounts in solution used for both experiments (a and b) were 1) 0.0µg 2) 0.025µg 3) 0.05µg 4) 0.1µg 5) 0.5µg 6) 1µg 7) 2µg 8) 3µg 9) 6µg 10) 8µg. Gel images were obtained using GelDoc Software.