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. Author manuscript; available in PMC: 2013 Apr 9.
Published in final edited form as: Biomacromolecules. 2012 Mar 14;13(4):974–981. doi: 10.1021/bm300083e

Figure 3.

Figure 3

Representative characterization data of purified E279C. A: DLS measurement of E2-WT (26.6 ± 0.6 nm, n=3, red dashed line) and E279C (28.0 ± 0.8 nm, n=3, blue solid line). B: Molar ellipticity versus wavelength shows characteristic minima at 208 and 222 nm for E2-WT and E279C, indicative of high alpha-helical structure. C: Thermostability profile (50–95 °C) measuring ellipticity at 222 nm. The calculated average To and Tm for E279C are 80.0 ± 1.5 °C and 89.5 ± 1.9 °C, respectively (n=3).