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. 2012 Apr 3;6:13. doi: 10.3389/fncel.2012.00013

Figure 2.

Figure 2

CRH is located in GABAergic axonal terminals. (A) CRH-ir axon terminals form dense perisomatic baskets (arrows) around pyramidal cells in hippocampal area CA3. (B) CRH immunoreactivity localizes to pre-synaptic terminals, demonstrated by dual-labeled immunocytochemistry for CRH and for the pre-synaptic marker syntaxin. (C,D) CRH-containing terminals are GABAergic boutons as indicated by overlapping immunoreactivities of CRH and GAD65 (C) and of CRH and the GABA synaptic vesicular transporter VGAT (D) in axon terminals. (E) CRH-ir axon terminals (at) form axosomatic symmetric synapses (arrowhead) with pyramidal cell body (soma). (F) CRH-ir axon terminals form axodendritic asymmetric synapses (arrowhead) with dendrites (dend). Empty arrow indicates a bouton containing CRH-negative vesicles. (G) Electron micrograph demonstrating CRH-ir gold particles (arrow) within the dendrite, but not within vesicles. Scale bars = 50 μm (A), 10 μm in (D) (25 μm for B and 12.5 μm for C), and 0.2 μm (E–G). Reproduced, with permission, from Chen et al. (2004b) (B–D) and Yan et al. (1998) (E).