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Microscopy was used to visualize location of the PEI-ce6 in association with C. neoformans. Cells were grown at 37°C, exposed to PEI-ce6 for 10 min and then washed with PBS. Arrows indicate localization of the PEI-ce6 at the cell wall perimeter. Arrowheads mark the localization of PEI-ce6 within the interior of the cell. With wild-type cells (KN99α), the dye was confined to the surface of the cells. However, with the KN99α rom2 mutant, the dye was able to penetrate the cell surface.
