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. 2012 Apr 1;2012:342854. doi: 10.1100/2012/342854

Figure 2.

Figure 2

Percentage of CD14-derived proinflammatory cytokine in OA knee patients. (a) An electronic gate was made for CD4−/CD14+ single positive cells. (b) From the gate a CD4−/CD14+/IL-1β+ cells were determined. (c) Percentage of CD4−/CD14+/IL-1β+ peripheral blood cells was established at baseline, 3, and 6 months. (d) An electronic gate was made for CD4−/CD14+ single positive cells. (e) From the gate d CD4−/CD14+/TNF-α+ cells were determined. (f) Percentage of CD4−/CD14+/TNF-α+ peripheral blood cells was established at baseline, 3, and 6 months. (g) An electronic gate was made for CD4−/CD14+ single positive cells. (h) From the gate f CD4−/CD14+/IL-10+ cells were determined. (i) Percentage of CD4−/CD14+/IL-10+ peripheral blood cells was established at baseline, 3 and 6 months. (j) An electronic gate was made for CD4+/CD14− single positive cells. (k) From the gate j CD4+/CD14−/Foxp3+ cells were determined. (l) Percentage of CD4+/CD14−/Foxp3+ peripheral blood cells was established at baseline, 3, and 6 months. (m) An electronic gate was made for CD8+/CD28− single positive cells. (n) From the gate m CD8+/CD28−/Foxp3+ cells were determined. (o) Percentage of CD8+/CD28−/Foxp3+ peripheral blood cells was established at baseline, 3, and 6 months. The software employed was CellQuest (BD Biosciences). A total of 50,000 events were recorded for each sample. Results are expressed as mean ± SE. *P < 0.05.