FIG. 5.

Expression and chemotactic activity of LTN protein expressed by plasmids with various combinations of LTN and VP1 genes. (A) 293T cells were transfected with indicated plasmids with lipofectamine for 48 h, and then cell lysates were subjected to western blot analysis using anti-LTN and anti-VP1 antibodies, respectively. (B) Meanwhile, supernatants were collected and subjected to in vitro chemotaxis assays, which were conducted using a modified 48-well Boyden chamber migration assay. *p<0.05. The results are represented as the mean±SD of three separate experiments.