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. 2010 Jul 1;9(13):2640–2646. doi: 10.4161/cc.9.13.12120

Figure 4.

Figure 4

Overexpression of Senp2 resulted in changes of SUMO modified proteins. (A) Myc-Senp2 protein was expressed in mouse oocytes. Myc-Senp2 mRNA microinjected GV oocytes were incubated in M16 medium containing 2.5 µM milrinone for 5 h and collected immediately; the lysates were then analyzed by immunoblot with mouse monoclonal anti-myc antibody. Equal numbers of oocytes microinjected with Myc mRNA was analyzed as a control. (B) Germinal vesicle oocytes were microinjected with the control Myc mRNA and Myc-Senp2 mRNA, respectively. After microinjection, oocytes were incubated in M16 medium with 2.5 µM milrinone for 5 h, then transferred to milrinone-free M16 medium for 10 h and collected, followed by immunoblot analysis with SUMO-1 or SUMO-2/3 specific mouse monoclonal antibodies. Three independent experiments were performed.