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. 2012 Apr 1;2012:141240. doi: 10.1100/2012/141240

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Copyright © 2012 Tatjana L. Rothhaar et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Determination of γ-secretase activity in the presence of PC-PL 18 : 1, PC-PL 20 : 4, PC-PL 22 : 6, and PE-PL 22 : 6. (a) Influence on γ-secretase activity in purified membranes of human SH-SY5Y cells. Purified membranes of SH-SY5Y cell were incubated with PC-PL 18 : 1, 20 : 4, 22 : 6, or PE-PL 22 : 6 and the corresponding phospholipids (100 μM), and γ-secretase activity was determined by a fluorometric assay. (b) Influence on γ-secretase activity ex vivo in purified membranes of mouse brains. Purified mouse brain membranes were incubated with PC-PL 18 : 1 and PC-PL 20 : 4 and the corresponding phospholipids (100 μM), and γ-secretase activity was determined. (c) Cultured SH-SY5Y cells were incubated with PC-PL 18 : 1 and PC-PL 20 : 4 and the corresponding phospholipids PC 18 : 1 and PC 20 : 4 for 24 hours in a final concentration of 100 μM. Membranes of incubated SH-SY5Y cells were prepared and γ-secretase activity was determined with a fluorometric assay. (a, b, c) Representative kinetics are shown for PC-PL 18 : 1 and PC 18 : 1. All quantified data represent an average of at least three independent experiments. Illustration and statistical significance are as described for Figure 2.