Skip to main content
. 2012 Apr 10;3:65. doi: 10.3389/fphys.2012.00065

Figure 11.

Figure 11

Visualization of the G-protein subunit Gαq and Gαi2 in G-cells. Double-labeling experiments employing antibodies for either Gαq (green) and gastrin [red (A–C) and (G–I)] or Gαi2 (green) and gastrin [red (D–F)] on horizontal tissue sections through the antrum of mouse or the human antrum. (A,G) Gαq-positive cells in the gastric antrum of mouse and men. (B,H) In the same area numerous G-cells can be visualized with the gastrin antibody. (C,I) The overlay of accordant images reveals the coexpression of the G-protein subunit Gαq and gastrin in murine (C) and human G-cells (I). (D) Numerous Gαi2-positive cells in the murine antrum mucosa. (E) Gastrin-positive cells are located in the same antral region. (F) Overlay of (D,E); murine G-cells coexpress the G-protein subunit Gαi2. Sections are counterstained with DAPI (blue). Scale bars: (A–I) = 20 μm.