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. 2012 Apr 10;3:65. doi: 10.3389/fphys.2012.00065

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Copyright © 2012 Haid, Jordan-Biegger, Widmayer and Breer.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Reverse transcriptase polymerase chain reaction analysis for GPRC6A, CaSR, and ribosomal protein l8 (Rpl8) mRNA from human (A) and porcine (B) antrum, respectively. Reverse transcription polymerase chain reaction (RT-PCR) experiments were performed with primer pairs specific for hGPRC6A (388 bp), hCaSR (251 bp), and hRpl8 (292 bp), as well as for sGPRC6A (287 bp), sCaSR (330 bp), and sRpl8, respectively. With cDNA from human or porcine antrum tissue distinct bands of the expected molecular size could be amplified. No bands were observed in water controls lacking template.