Figure 4.

Visualization of GPRC6A in G-cells of mouse, swine, and human. Double-immunolabeling of horizontal tissue sections through the antral mucosa of mouse, swine, and human for GPRC6A (green) and gastrin (red). (A,D,G) GPRC6A-positive cells in antral annuli of mouse (A), swine (D), and men (G). (B,E,H) In the same annuli numerous gastrin-positive cells can be visualized. (C,F,I) The overlay of accordant images clearly reveals that the vast majority of G-cells is immunoreactive for GPRC6A in the three species. Sections are counterstained with DAPI (blue). Scale bars: (A–I) = 20 μm.