Table II. IPA network analysis of differentially expressed plasma proteins in Chagasic rats. Sprague Dawley rats were infected with T. cruzi and treated with phenyl-α-tert-butyl nitrone (PBN) and benznidazole (BZ) as detailed in Materials and Methods. Plasma samples were collected at day 25 (acutely infected) and 150 (chronically infected) post-infection, and subjected to 2D-gel electrophoresis/mass spectrometry for identification of the differentially expressed proteins. All differentially expressed proteins (listed in Table I) were uploaded into Ingenuity Pathway Analysis (IPA) to interpret datasets in the context of biological processes and function, and pathway and molecular networks. Presented are the top three networks with a score [-log(p value)] of 25 or greater to which maximal number of the differentially expressed proteins identified in chagasic plasma (bolded) were associated with. Focus molecules are the number of differentially expressed plasma proteins associated with individual network.

ID	Molecules in network	Score	Focus molecules	Top functions
1	Actin, ALB, APOA1, APOA4, APOE, APOH, C3, CFB, CFH, CFHR1, CFP, CHD4, Cytokeratin, DSTN, FGB, Fibrinogen, GSN, HPX, IgG, KRT1, KRT3, KRT4, KRT10, KRT12, KRT13, KRT23, KRT6B, PLG, SERPINF, SERPINF1, SETX, TF, TMPRSS6, TRY6, VIM	41	19	Antigen Presentation, Humoral Immune Response, Inflammatory Response
2	AFM, APP, BRPF3, C4BPA, CACNA1D, COL25A1, DLGAP2, GAB1/2, GC, GRB2, heparin, INSR, KCNMA1, KNG1 (includes EG:16644), Met dimer, MUG1, MYLPF, MYO5A, MYO5B, MYO5C, PFKFB2, PIK3AP1, PIK3R1, PLA2G2D, PLA2G2E, PRPH, PZP, RAI14, SCG2, SERPINC1, SLC23A1, SNX8, VPS13A, YWHAZ, ZNF32	36	17	Lipid Metabolism, Molecular Transport, Small Molecule Biochemistry
3	ATG4C, BFAR, C1ORF25, CASP8, CEBPB, CEP350, CIDEC, CRAT, DSCR3, ESR1, GNAQ, GPT, HNF4A, IDH3A, ITIH4, LGMN, MINA, MYC, PEPD, PPARG, PUS1, PUS3, SCD2, SERPINA1, SLC25A19, SQRDL (includes EG:58472), SRPRB,SYMPK, TMEM176A, TMEM176B, TRUB2, TTC37,UMPS, YME1L1, ZNHIT6	29	14	Cellular Development, Free Radical Scavenging, Molecular Transport