Fig. 4.

Ydl156w exists in high molecular weight complexes. The yeast protein Ydl156w was purified from S. cerevisiae and fractionated by size exclusion chromatography on a superose 6 HR 10/30 column. A, Fractions were collected and aliquots were TCA precipitated, separated using SDS-PAGE and transferred to nitrocellulose membranes for Western blotting. The TAP-tagged Ydl156w was detected with polyclonal anti-TAP IgG. B, MudPIT analyses were carried out on superpose six fractions and dNSAF values of the 17 proteins identified in fraction 5, as well as their relative abundance levels in fraction 23 are shown.