Fig. 2.

Plasma membrane enrichment. A, immunoblot analysis of aqueous two-phase partitioning PM enrichment. Total leaf, total microsome, and PM-enriched fractions are probed with antibodies raised against the indicated proteins. Established protein subcellular localization is indicated within parentheses. ER, endoplasmic reticulum; BIP, luminal-binding protein 1; PED1, peroxisome defective 1; TOC75, translocon at the outer envelope membrane of chloroplast 75; alpha-TIP, tonoplast intrinsic protein α; VDAC, voltage-dependent anion channel; RIN4, RPM1-interacting protein 4. Ten μg of total protein was loaded per lane. B, in silico localization of all proteins identified by LC-MS/MS. The proteins were queried using the subcellular location database for Arabidopsis proteins (SUBA 2.21).