Fig. 3.

Association of DEAD/H proteins with Rev by co-immunoprecipitation. A, Schematic representation of the pGagRRE and pGagCTE constructs used in the analysis. pGagRRE contains a fragment of the the env gene with the 351 nt RRE, whereas pGagCTE instead contains four copies of the constitutive transport element (CTE). B, Western blot analysis to detect the indicated DEAD/H helicases in whole cell lysates of transfected cells and in immunoprecipitates derived from these with anti-V5 antibody or with control IgG. Cells were transfected with pRev-V5 and one of the two plasmids described in panel A. Where indicated, cell lysates were pretreated with RNase A. Fifteen sample equivalents were loaded on the immunoprecipitate lanes, as compared with one equivalent for the input lanes (C) Western blot analysis of whole cell lysate with anti-p24 and anti-V5.