Problem Handling.

Problem	Possible reason	Solution
Catheter Placement, Inoculation Removal
Rabbit Restraint: Rabbits can be unpredictable	Rabbits are usually timid and are easily upset.	Lift the rabbit firmly by the scruff with the head pointing away from your body, use your other hand to support the rabbit's hind end. Cradle the rabbit to your body. If the rabbit struggles, place it on a solid surface and calm it by shielding the rabbit's eyes by tucking its head into the crook of your arm; this should keep the animal calm. When done, keep a firm grip on the scruff and gently release the rabbit in to the correctly marked cage.
Scanning Electron Microscopy (Adapted from Chandra et al.5)
Catheter segments too thick and Candida biofilms too dense	Catheter segments not cut flat and uniform and there is overproduction of Candida biofilm on the segments.	Focus on areas where biofilms are spread out or on edges of the segment, and catheter segments are flat Evaluate cells at earlier time point.
“Burnt” images	Sputter-coating step too long Exposing the cells to electron beam for extended period	Check sputter coating setting. Take images quickly. Use lower magnification to scan the image, then capture image rapidly at higher magnification.
Blurry/out of focus	Insufficient sputter coating Instrument setting not correct	Re-sputter coat sample. Ensure the instrument is focusing correctly. Make sure this problem is not user-dependent.