Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Struct Biol. 2012 Feb 16;177(3):630–637. doi: 10.1016/j.jsb.2012.02.003

Figure 3.

Figure 3

Movie frame averages of rotavirus DLPs in ice on a Quantifoil® grid (hole size = 1.6 μm). The movie was recorded for 1.5 sec at 40 frames/sec using the DE-12 direct electron detector (Direct Electron). 10-frame averages of the resulting 60 frames were subjected to the same analysis as the exposure series. Vector plots in panels A – E indicate particle rotations measured between frame averages. The total average of the movie is shown in the background in A and the summed rotations from panels A – E are shown in F. Panels G – K show vector plots indicating the particle translations measured between frame averages. Panel L shows the summed translations from panels G – K. The square highlights the area shown as a close-up in Figure 5. The movie is further analyzed in Supplementary material (Movies S1 – S3).