Table A2.
Primers and annealing temperatures used in this study.
| Primer | Target gene | Primer sequence (5′–3′) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
| sul1-Fw | sul1 | CGCACCGGAAACATCGCTGCAC | 69.9 | Pei et al. (2006) |
| sul1-Rv | TGAAGTTCCGCCGCAAGGCTCG | |||
| sul2-Fw | sul2 | TCCGGTGGAGGCCGGTATCTGG | 67.5 | Pei et al. (2006) |
| sul2-Rv | CGGGAATGCCATCTGCCTTGAG | |||
| erm(B)-Fw | erm(B) | GATACCGTTTACGAAATTGG | 58 | Chen et al. (2007) |
| erm(B)-Rv | GAATCGAGACTTGAGTGTGC | |||
| erm(F)-Fw | erm(F) | CGACACAGCTTTGGTTGAAC | 56 | Chen et al. (2007) |
| erm(F)-Rv | GGACCTACCTCATAGACAAG | |||
| tet(C)-Fw | tet(C) | GCGGGATATCGTCCATTCCG | 70 | Aminov et al. (2002) |
| tet(C)-Rv | GCGTAGAGGATCCACAGGACG | |||
| tet(G)-Fw | tet(G) | GCAGAGCAGGTCGCTGG | 64.2 | Aminov et al. (2002) |
| tet(G)-Rv | CCYGCAAGAGAAGCCAGAAG | |||
| tet(O)-Fw | tet(O) | ACGGARAGTTTATTGTATACC | 50.3 | Aminov et al. (2001) |
| tet(O)-Rv | TGGCGTATCTATAATGTTGAC | |||
| tet(W)-Fw | tet(W) | GAGAGCCTGCTATATGCCAGC | 60 | Aminov et al. (2001) |
| tet(W)-Rv | GGGCGTATCCACAATGTTAAC | |||
| tet(X)-Fw | tet(X) | CAATAATTGGTGGTGGACCC | 64.5 | Ng et al. (2001) |
| tet(X)-Rv | TTCTTACCTTGGACATCCCG | |||
| HS463a | intI1 | CTGGATTTCGATCACGGCACG | 60 | Hardwick et al. (2008) |
| HS464 | ACATGCGTGTAAATCATCGTCG | |||
| 1369F | 16S rRNA | CGGTGAATACGTTCYCGG | 60 | Suzuki et al. (2000) |
| 1492R | GGWTACCTTGTTACGACTT |