Echocardiography (ECHO) was performed at Baseline, Day 7 (during injury), Day 10 (end of injury phase), Day 17 (early recovery) and Day 38 (late recovery). Representative ECHO data is shown in (A). B–D: Cardiac systolic and diastolic function was increasingly depressed from Baseline through Day 10 (N=19) and showed modest improvement after removal (at Day 10) of ISO minipumps by Day 17 (N=12) and at Day 38 (N=6). The transmitral E to A ratio was significantly depressed, indicating diastolic stiffening of the heart at Days 7 (N=15), 10 (N=17) and 17 (N=9), with modest recovery at Day 38 (N=6). *P<0.05; **P<0.01; ***P<0.001 versus Baseline. Data are presented as mean ± SEM.