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. Author manuscript; available in PMC: 2012 May 13.
Published in final edited form as: Circ Res. 2011 Mar 31;108(10):1226–1237. doi: 10.1161/CIRCRESAHA.110.239046

Figure 5. ISO injury increases the number of BrdU+ non-myocytes during injury and the number BrdU+ myocytes in the atria after injury.

Figure 5

BrdU was infused for 7 days before euthanasia, except in pulse-chase studies where it was infused during injury (Pulse) and animals were euthanized at Day 38 (Chase). BrdU (Red); α-Actin (Green); DA PI (Blue). Few BrdU+ nuclei were observed in normal atria and most of these were non-myocyte nuclei (A/D/E). ISO induced injury caused a large increase in the number of BrdU+ nuclei (Day 10; B) but almost all of the BrdU+ nuclei were non-myocytes (D/E). The total number of BrdU+ nuclei decreased rapidly after removal of ISO minipumps, at Day 17 (C/D) and Day 38 (D). The number of BrdU+ myocyte nuclei increased at Day 17 (C/E) but was back to control levels by Day 38 (E). BrdU+ non-myocytes labeled during the injury phase (Pulse) persisted in the heart for 4 weeks (Day 38; Chase) and the largest number of BrdU+ myocytes were found when labeling was done at this time period (E). *P<0.05; **P<0.01; ***P<0.001 versus Control. Data are presented as mean ± SEM.