Figure 5.

Day 6. Histology of longitudinal ground sections of MS (a) and SLA (b) surfaces. Empty osteocytic lacunae (arrows). LM, toluidine blue, and acid fuchsin staining. Bar = 100 μm. Scanning electron microscopy of deacrylated ground sections of SLA implants: spreaded polygonal osteoblast-like cells (c) and osteoclast-like cell presenting a ruffled border (d) adherent to the metal surface. Since the samples were initially processed for light microscopy (buffered formalin fixative) and afterwards deacrylated for SEM observation, the shrinked appearance of blood cells might be considered as an artifact due to the formalin fixation.