FIGURE 1.

Knockdown of select genes of the two pathways in DCs results in decreased survival of M. tuberculosis. DCs were transfected with siRNAs against indicated genes belonging to either the calcium calmodulin pathway or cysteine protease pathway for 72 h, followed by infection with 2.5 MOI M. tuberculosis H37Rv for 48 h. Cell lysates were plated onto 7H11 agar plates, and cfu were monitored after 2–3 weeks. Mock represents knockdown with a control siRNA. Data represent mean ± S.D. of three independent experiments. *, p = 0.012 for Mock versus Camkiia; **, p = 0.017 for MOCK versus Usp25; ***, p = 0.006 for Mock versus Ctsh; and ****, p = 0.016 for Mock versus Snrk. TTN, titin.