FIGURE 6.

Knockdown of Camkiia or Usp25 or Senp8 increases antigen- and receptor-mediated Beclin-1 and ATG5 expression. A, DCs were transfected with siRNAs against the indicated genes, followed by costimulation with 5 μg/ml manLAM and 15 μg/ml Rv2463 for 4 h. Cells were incubated with antibody to Beclin-1 (A) and followed by staining with anti-rabbit FITC-tagged Alexa Fluor 488 (B). Images were captured under a confocal microscope. Bars below each panel represent the expression levels quantified as average of sum intensity of each fluorescent point by using NIS Elements AR software. siControl represents knockdown with a control siRNA. Data from one of three experiments are shown.