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Knockdown of select genes induces autophagy in M. tuberculosis-infected DCs. DCs were transfected with siRNAs against indicated genes, followed by infection with 2.5 MOI M. tuberculosis (M. tb) H37Rv for 24 h. Cytoplasmic extracts were Western blotted for Beclin-1 and ATG5 expression. MOCK represents knockdown with a control siRNA. Numbers below the Beclin-1 or ATG5 blots indicate relative intensities of the bands. Data from one of two experiments are shown.
