FIGURE 9.

Knockdown of select gens enhances ROS generation in M. tuberculosis-infected DCs. DCs were transfected with siRNAs against indicated genes and later infected with 2.5 MOI M. tuberculosis H37Rv for indicated times. 30 min prior to the incubation period, cells were loaded with 10 μm DCFH-DA. At the end of the incubation period, cells were quickly and thoroughly washed with culture medium and fixed with 4% paraformaldehyde for 2 h. Cells were washed once again with culture medium and immediately analyzed for ROS levels by flow cytometry. Mock represents transfection with control siRNAs. Black lines depict ROS levels in DCs transfected with control siRNAs. Green lines depict DCs transfected with gene-specific siRNAs. Data from one of two experiments are shown.