TABLE 1.
Knockdown of specific genes modulates surface densities of costimulatory molecules on DCs
The indicated genes were knocked down in DCs with specific siRNAs followed by stimulation with either Pam3Csk4 (Pam) or manLAM along with Rv2463 or Rv3416, as indicated. The surface levels of indicated markers were monitored by flow cytometry. Data are represented as increase (+) or decrease (−) over that observed in control siRNA transfected and similarly stimulated DCs. Number of + or − indicates levels of increase or decrease, respectively. Data are representative of two independent experiments.
| Gene/stimulation | CD80 | CD86 | CD40 | CD54 |
|---|---|---|---|---|
| SNRK | ||||
| Rv2463+Pam | ++ | +/− | +/− | +/− |
| Rv2463+manLAM | +/− | +/− | +/− | +/− |
| Rv3416+Pam | +/− | +/− | +/− | +/− |
| Rv3416+manLAM | + | + | + | +/− |
| USP25 | ||||
| Rv2463+Pam | +/− | +/− | +/− | +/− |
| Rv2463+manLAM | + | +/− | +/− | +/− |
| Rv3416+Pam | − | +/− | +/− | + |
| Rv3416+manLAM | ++ | + | + | +++ |
| SENP8 | ||||
| Rv2463+Pam | + | +/− | +/− | +/− |
| Rv2463+manLAM | +/− | +/− | +/− | +/− |
| Rv3416+Pam | − | +/− | +/− | +/− |
| Rv3416+manLAM | + | + | + | +++ |