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. 2012 Feb 3;287(14):10922–10932. doi: 10.1074/jbc.M111.333393

FIGURE 2.

FIGURE 2.

IF2 binds GDP through a direct hydrogen bond. The figure shows the data of the direct detection of intermolecular JNP scalar couplings. One-dimensional 31P–15N spin-echo difference 15N-HSQC spectrum of IF2-G2·GDP and a schematic view of the phosphate-amide proton hydrogen bonds are detectable with the pulse sequence of supplemental Fig. S2.