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Deacylation of charged tRNA by M. mobile LeuRS and hybrid mutants. A and B, deacylation of 2 μm misaminoacylated Ile-tRNALeu (A) or Met-tRNALeu (B) by 1 μm M. mobile LeuRS wild type or hybrid proteins that contain an E. coli CP1 domain. Symbols used are as follows: ×, nonenzymatic tRNA deacylation (no E); ■, MmLeuRS (wild type); △, MmLeuRS/CP1Leu; ▿, MmLeuRS/CP1Ile; ◊, MmLeuRS/CP1Val. Error bars represent the S.D. values based on three separate experiments.
