TABLE 2.
Conserved active site residues in AfUGM compared to prokaryotic UGMs
The OH group of Tyr-317 makes the same hydrogen bonding interaction with the α-phosphate of the nucleotide as OH group of Tyr-209 in DrUGM and Tyr-185 in KpUGM (single letter amino acid abbreviations are indicated).
| AfUGM | DrUGM | KpUGM | EcUGM | MtUGM |
|---|---|---|---|---|
| H63 | H85 | H60 | H56 | H65 |
| F66 | H88 | H63 | H59 | H68 |
| R91 | H109 | N84 | N80 | H89 |
| F158 | F175 | F151 | L147 | F157 |
| M159 | F176 | F152 | I148 | V158 |
| Y162 | Y179 | Y155 | Y151 | Y161 |
| N163 | T180 | T156 | T152 | T162 |
| W167 | W184 | W160 | W156 | W166 |
| R182 | R198 | R174 | R170 | R180 |
| P206 | Y209 | Y185 | Y181 | Y191 |
| N207 | F210 | F186 | F182 | F192 |
| W315 | T294 | V268 | V266 | T162 |
| Y317 | N296 | N270 | N268 | N282 |
| R327 | R305 | R280 | R278 | R292 |
| E373 | E325 | E301 | E298 | E315 |
| Y419 | Y335 | Y314 | Y311 | Y328 |
| R447 | R364 | R343 | R340 | R360 |
| Y453 | Y370 | Y349 | Y346 | Y366 |
| N457 | N372 | D351 | D348 | D368 |
| Q458 | M373 | M352 | M349 | M369 |