The class I/II histone deacetylase inhibitors (HDI) TSA induces survivin acetylation in ER+ breast cancer cells.
A, acetylated survivin Lys-129 and total survivin levels in response to nicotinamide (NAM), TSA, or both in MCF-7 cells, as determined by Western blotting using a specific antibody to acetylated survivin Lys-129 (Survivin129Ac) or total survivin, respectively. B, dose response of TSA-mediated survivin Lys-129 acetylation in MCF-7 cells. C, time course of survivin Lys-129 acetylation in 1 μm TSA-treated MCF-7 cells. D, subcellular localization of acetylated and total survivin in MCF-7 cells treated with TSA or dimethyl sulfoxide (DMSO; vehicle control) for 6 h and then fixed and co-immunostained with antibodies to acetylated survivin and total survivin (green and red, respectively) and DAPI. Images were taken with a Nikon confocal microscope. Scale bar, 20 μm.