FIGURE 4.

Survivin deacetylation by HDAC6 is independent of the nuclear export protein Crm1. A, untreated HeLa cells were lysed and immunoprecipitated with anti-HDAC6 or immunoglobulin control (Ig) and immunoblotted with anti-Crm1 and anti-HDAC6. Total Crm1 and total HDAC6 protein are shown as input. B, HeLa cells were co-transfected with FLAG-HDAC6, HA-CBP, and Myc-survivin then treated with or without LB, as described previously (8). Total cellular lysates were immunoblotted (IB) with anti-acetylated survivin, anti-Myc, and anti-FLAG. C, HeLa cells were co-transfected with expression plasmids encoding FLAG-HDAC6, HA-CBP, or an empty vector control (EV) and then treated with LB as described previously (3). Cells were fixed and immunostained with anti-survivin (red), anti-HDAC6 (green), and DAPI and imaged by a Nikon confocal microscope. Scale bar, 20 μm. IP, immunoprecipitation.