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. 2012 Feb 23;287(14):10958–10966. doi: 10.1074/jbc.M112.345041

FIGURE 7.

FIGURE 7.

His-Asp phosphorelay. A, in vitro phosphorylation assay with GHK4-K and FgrM-R. GHK4-K and FgrM-R were tested for autophosphorylation and phosphotransfer activity in vitro. The molecular mass standards are shown in kilodaltons. B, model for phosphorelay. HK, histidine kinase; REC, receiver domain; AAA/Sigma54_activat, AAA/σ54 activation domain. C, in vitro phosphorylation assay with GHK3-K and GHK4-R. GHK3-K and GHK4-R were tested for autophosphorylation and phosphotransfer activity in vitro. The molecular mass standards are shown in kilodaltons.