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. 2012 Feb 7;287(14):11030–11036. doi: 10.1074/jbc.M111.334094

FIGURE 3.

FIGURE 3.

HEAT repeat 1 mutants of B56γ fail to inhibit cell proliferation. A, lysates of U2OS cells transfected with HA-tagged wild type B56γ (WT), C39R, C39A, C39S, ΔN40, ΔN53 and ΔN73, or a control empty vector (EV) were analyzed by Western blot for p53 Thr-55 dephosphorylation (Thr55 Phos), p21, HA-B56γ, p53, and vinculin. B, representative data of cell proliferation of the HCT116 human colon cancer cell lines transfected with HA-tagged wild type B56γ, C39R, ΔN40, or ΔN73, or a control empty vector. The number of cells present at the 120 h time point was normalized against the empty vector controls and plotted in a bar graph. Error bars show average ± S.E. from triplicate plates in one representative experiment. Cell lysates were analyzed by immunoblotting of transfected HA-B56γ and p53 proteins.