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. 2012 Feb 7;287(14):11030–11036. doi: 10.1074/jbc.M111.334094

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — HEAT repeat 1 mutants of B56γ fail to inhibit anchorage-independent cell growth. A, representative data of anchorage-independent growth of HCT116 human colon cancer cell lines transfected with HA-tagged wild type B56γ (WT), C39R, or ΔN40 or a control empty vector (EV). B, colony numbers for anchorage-independent growth were counted and represented on a bar graph. The values are the averages ± S.E. from three experiments. Cell lysates were analyzed by immunoblotting of transfected or endogenous B56γ, p53, and vinculin proteins.