FIGURE 4.
Reduced NCR247 shows increased antimicrobial activity against S. meliloti. A, RP-HPLC analysis of NCR2471–2,3–4 treated with (solid line) and without (dashed line) 10 mm DTT for 5 min in sodium phosphate buffer. B, ESI-MS analysis of the peak eluted between 24.7 and 25.5 min, consistent with a fully reduced NCR247 peptide. C, ESI-MS analysis of the peak eluted between 26.7 and 27.2 min, consistent with the folded, oxidized form of NCR247. D, colony forming ability of S. meliloti Sm1021 assessed after treatment with 10 μm NCR2471–2,3–4 and NSR247 peptide for 3 h in the presence and absence of DTT. The dataset shown is representative of trends observed in two independent experiments. Columns represent mean ± S.E. (error bars) (n = 3), and significance was determined with one-way ANOVA and Bonferroni's post test on logarithmic data. ***, p ≤, 0.001.