FIGURE 5.
NCR247 folding is important for BacA-mediated protection. A–C, colony forming ability of S. meliloti Sm1021 parent and the BacA-deficient mutant assessed after peptide treatment. A, 10 μm NCR2471–2,3–4 and NCR2471–3,2–4. B, 5 μm NCR2471–2,3–4 without (oxidized) and with (reduced) 1 mm DTT. C, 20 μm NCR2471–2,3–4 and NSR247. The datasets shown are representative of trends observed in two independent experiments each. Columns represent mean ± S.E. (error bars) (n = 3). Significance was determined with one-way ANOVA and Bonferroni's post test; ns, not significant. *, p ≤ 0.05; **, p ≤ 0.01; ***, p ≤ 0.001.