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. 2004 Feb;10(2):179–184. doi: 10.3201/eid1002.030677

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Amplification of severe acute respiratory syndrome–associated coronavirus (SARS-CoV) RNA in chicken blood, using one-step and two-step reverse transcriptase–polymerase chain reaction (RT-PCR) with nucleocapsid primers. Lane 1: 100-bp ladder, the bright band representing 600 bp; Lane 2: chicken 115, 2 days postinocuation (dpi), one-step RT-PCR; lane 3: chicken 115, 2 dpi, two-step RT-PCR (detecting negative-strand RNA); lane 4: chicken 117, 3 dpi, one-step RT-PCR; lane 5: chicken 117, 3 dpi, two-step RT-PCR; lane 6: chicken 115, 4 dpi, one-step RT-PCR; lane 7: chicken 115, 4 dpi, two-step RT-PCR; lane 8: SARS-CoV–infected cells; lane 9: negative control.