FIGURE 5.

[³H]NA secretion was modulated by PRIP-1. PC12 cells expressing GFP (A), GFP-PRIP-1 (WT) (B), GFP-RPIP-1 (F97A) (C), or GFP-PRIP-1 (T94A) (D) were labeled with [³H]NA, followed by stimulation with 50 μm FSK for 5 min. After removal of the stimulus, cells were left at room temperature for 5, 10, 20, or 30 min, followed by [³H]NA secretion assay with high K⁺ solution for 5 min. All the data are the means ± S.E. (error bars) of five (A–C) or three (D) experiments.